About G6S

What is G6S?

I decided to put a little bit of information on G6S testing as for the first time I had a buyer requesting this test done on a buck kid. I had never felt the need for testing before but since this request I figured I would start testing my bucks, perhaps it was the push I needed to get it started. I was actually more intimidated by the complicated process to get it done and also the upfront cost.

A great friend of mine, the late Dagny Vidinsh had written this article in 2001. First I have posted Dagny's article but also the steps to take to get the testing done here in Canada.

 



Look for the following logos on our animals pedigrees to see their G6S Status if they have been tested.




 

A Genetic Defect and its Management
by Dagny Vidinsh

All animals, including dairy goats, have numerous genetic defects of varying severity. We are all familiar with the occasional multiple teats, for instance, and with such defects as undershot and overshot jaws. Other defects are rapidly fatal, and it often is unclear whether the death of a kid should be attributed to genetics or to misfortune. The exact inheritance of many of these defects is often obscure; for instance, although most people believe that multiple teats show up when both parents carry a gene for this trait there is evidence that in some cases they are actually caused by environmental factors. In order to manage these undesirable genes breeders usually have to fall back on the "don't repeat that breeding" strategy, which is very crude and unsatisfactory.

This article will describe a recently discovered genetic defect which is easily managed and eliminated because it's mode of transmission is straightforward and, more important, because a foolproof DNA test is available to identify carriers of the gene. This defect's full names are mucopolysaccharidosis IIID, or G-6-Sulfase deficiency, and it is usually referred to as G-6-S. It was first identified in 1987 at Michigan State University, and subsequently the researchers tested nearly a thousand goats in Michigan and concluded that about 25% of Nubians carry this gene. All cases are the result of a single mutation, and appear to be confined to Nubians and their crosses; other breeds were tested initially and they do not have this particular defect. The affected goats lack an enzyme (G-6-S) and this results in a variety of symptoms of varying severity. The main symptom exhibited by affected goats is failure to grow. Sometimes the kid is smaller than normal at birth, and grows slowly. Some breeders have reported kids which grew normally for the first three months and then stopped growing. Other affected goats grow to what appears to be normal size but is in fact small for the particular bloodlines. They lack muscle mass, appear "slab-sided", sometimes with blocky heads. Immune function appears to be compromised, and sometimes they become deaf or blind. The longest-lived goat known to be G-6-S affected died at just under four years of age, and death is usually due to heart failure. Unfortunately affected animals can and do grow up to breed, although they often experience reproductive problems. The same symptoms can have many other causes, so that affected animals are seldom recognized as having a genetic defect. Often they grow normally for the first few months and may be sold before any problems become apparent. In that case the breeder may blame the new owner for the goat's failure to thrive and early demise.

Every animal has two genes for every trait, one inherited from the dam and one from the sire. In turn, that animal will pass only one of those genes to each offspring, and which one it will be is a matter of chance, like the flip of a coin. On the average, half the offspring will inherit one gene and half the other. If the two genes are different, then there is a question as to which of them will determine how the animal actually looks or functions. The defective G-6-S mutation is a simple recessive gene, which means that a goat which has only one copy of it will appear perfectly normal and will not show any of the symptoms described above. Such a goat is referred to as a "carrier". A goat which inherits the defective gene from both parents shows symptoms and is referred to as "affected". A "normal" goat, in this context, is one who has two copies of the normal gene. If a normal goat is bred to a carrier, then all offspring will inherit a normal gene from the normal parent. The carrier parent will pass a normal gene to half the offspring, and a defective gene to the other half. Thus such a mating will, on the average, produce half normal kids and half carriers, and no affected ones. If two carriers are bred to each other, then one quarter of the kids will be normal, one half will be carriers, and one quarter will be affected. If an affected goat is bred to a normal goat, all offspring will be carriers. An affected goat bred to a carrier will produce half carriers and half affected.

As stated above, research shows that 25% of Nubians carry the defective G-6-S gene. Almost all of these are carriers, since most of the affected animals which are born would be culled, and the rest die early. Most people find it surprising that something which is in one quarter of the population can have escaped notice for so long. However, random matings in such a population would result in only one out of sixteen being carrier to carrier, and only one quarter of the kids from these breedings would be affected. Thus only one kid in sixty-four (1.6%) would be affected. Given the variable and obscure symptoms of G-6-S affected kids, it really is understandable that most Nubian breeders believe that they have never encountered affected kids. However, many Nubians are line-bred, and this practice will concentrate certain genes in some lines while eliminating them from others. It has been observed that the G-6-S mutation is very prevalent in the same lines which are known for high milk production. Thus breeders who have been selecting for milk may have inadvertently also been selecting for the G-6-S defect. Fortunately it appears that the two traits are actually independent, that you can cull the G-6-S carriers without at the same time culling the high producers. Usually it is difficult to eliminate a genetic defect without loosing all the good genetics for which a line is known. For instance, if a buck throws double teats, then there is no way of knowing which of his offspring will do the same and which will not. You can cull him, but that seems rather heavy-handed since the bad gene will undoubtedly live on in some of his relatives.

With G-6-S we are very fortunate to have a foolproof DNA test available which will tell us whether a goat is normal, or a carrier, or affected. This test makes it possible to save the good genetics and eliminate the defective gene if that is our wish. If a superior animal is a carrier, then we can test the kids and manage them in such a way as to avoid the birth of any affected individuals.

What is a good management strategy? What is the most efficient way to save the good and get rid of the bad? The usual recommendation for such testable defects is to cull carrier males, but not the females. Remember that if a normal buck breeds a carrier doe, then only half the kids will be carriers, and none will be affected. Thus if there are some carrier females in the herd, then using only normal bucks will reduce the incidence of carriers in the next generation by one half. The average herd would start with 25% carrier females, and if only normal bucks were used the next generation of females would be down to 12.5% carriers, and the next generation to 6.25%, etc. This is in sharp contrast to what a carrier buck would do in the same herd: if used to breed all the does, his daughters would be 50% carriers and 6.25% affected. Clearly there is much to be gained by testing buck kids and retaining only normal ones for breeding. While it is relatively easy to cull a buck kid, one might hesitate to do the same with a proven sire. In particular, there are some very popular bucks whose semen commands a high price and who are carriers for the defective G-6-S gene. A reasonable strategy here would be to use these bucks only on normal does, thus avoiding affected kids. Then one would test the kids and cull carrier bucks. Although the DNA tests are expensive, if testing one's bucks prevents the birth of even one affected kid then it is cost effective.

Unlike tests for diseases, a genetic test does not need to ever be repeated. Also, the DNA tests are completely accurate, there are none of the gray areas which can be so frustrating. There is no need to test the kids if both parents are known to be normal. One can work back from one's foundation animals and if there really is no problem in the herd then it may be possible to establish that at reasonable cost. Normally whole blood is used for the test, but semen can also be used. If an AI buck is a carrier, that can be established by finding a carrier offspring out of a normal doe, but no number of normal offspring will prove that a buck is normal.

A number of breeders have expressed the opinion that the G-6-S defect is no more of a problem than many other genetic defects, and therefore does not merit any particular attention. They evidently miss the point that it is the availability of a DNA test which makes this defect special. One can use goats from bloodlines which are known to have a high concentration of the G-6-S defect completely safely by just testing the particular individuals and either rejecting carriers or using them with proper precautions. There is nothing to be gained by trying to sweep G-6-S under a rug, and much to be gained by sharing information about it.

One may wonder why a DNA test has been developed for such an obscure defect, and no help is available for, say, multiple teats. The answer is simple- humans don't have a problem with multiple teats, they do with G-6-S. The same genetic defect, when found in humans, is called Sanfilippo IIID; the affected child appears normal at birth but soon stops growing, looses muscle mass, has neurological deterioration and dies. When the same genetic defect was discovered in goats researchers used them as models for treatment, and goat breeders in turn benefited from their discoveries.

Copyright Dagny Vidinsh, 2001
 


There is also this higher level article about G6S testing that is very interesting to read
http://vdi.sagepub.com/content/22/4/622.full.pdf

How to Test - Step by Step in Canada
Updated May 16th, 2012 - Please refer to the TVMDL website for the most up to date information on G6S testing, this is for informational purposes only, process may change without notice. The information below has been taken from the TVMDL website.

An accession (processing) fee of $7.50 for out-of-state tests will be charged for each accession. $42 per specimen.

1. It would be preferable to start by obtaining an import permit from TVMDL contact.
2. Fill out this test form and if this is your first test, you will also need to fill out the credit card payment information. This can also be done over the phone.
3. You'll need the Lavender EDTA vaccum tubes. These tubes have anti coagulant in it. The test requires 1 to 2 cc of blood or you can use two straws of semen.. Once the blood has been collected, store it in a cool place.
4. Here's a neat video on how to take a blood sample on a goat.

Packaging and Shipping Instructions:

It is very important to include the import permit as well as this exact Statement with the shipment or the package will not be accepted by customs!

Each shipment shall be accompanied by an ORIGINAL signed document from the producer/manufacturer confirming that the exported material:
1) was collected only from equine, bovine, ovine, caprine, canine, feline, lagomorph, and reptile species originating from Canada, and
2) was not exposed to any other animal origin material .
This certification must CLEARLY correspond to the shipment by means of an invoice number or shipping marks or lot number or other identification method.


TVMDL is also open on Saturdays from 8-12:00pm their time. It is preferable to ship the sample overnight in order to keep cool. Even if the shipper offers Saturday service, customs will not process your package on the weekend therefore I strongly advise against shipping on a Friday.

For Liquid Substances, see image of example packaging:
•The primary receptacle is leak-proof;
•The secondary packaging is leak-proof;
•Absorbent material is in sufficient quantity to absorb the entire contents placed between the primary and secondary packaging;
•If multiple fragile primary receptacles are placed in a single secondary packaging, they must be either individually wrapped or separated so as to prevent contact between them.

Classify
Category B infectious substance
TVMDL recommends all shipments be classified and shipped as a Biological Substance Category B unless the source patient or animal of concern has or may have a serious human or animal disease which can be readily transmitted from one individual to another, directly or indirectly, and for which effective treatment and preventable measures are not usually available.

Documentation
Air Waybill—The text “Biological Substance, Category B” and “UN 3373” should be documented on the waybill.

Ship to address:
Texas Veterinary Medical Diagnostic Laboratory
1 Sippel Road
College Station, TX 77843
Phone: 979-845-3414
Fax: 979-845-1794

Contact Information

Phone (979) 845-3414,
Toll Free (888) 646-5623
Fax (979) 845-1794
After-hours Cell Phone #979-324-4652
All other questions can be addressed by calling 888-646-5623.

Hours of Operation:
M-F 8:00AM-5:00PM,
Saturday morning specimen drop-off 8:00AM-12:00PM

Notes:
Don't ship for Saturday AM as the customs are closed even if the shipper promises on time delivery.
It would be preferable to use FedEx over Purolator as Purolator sends their shipment thru UPS when entering the USA.

In order to keep the blood samples cool, frozen gel packs should be added to the package. (No ice)

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Last Update: 10.30.2012